Anti-DAPK3 antibody [4H-C8]

Anti-DAPK3 antibody [4H-C8]

• 概述

• 产品描述DAP (death associated protein) kinase and ZIP kinase are members of a novel protein kinase family, the members of which have the capacity to mediate apoptosis through their catalytic activities. DAP kinase contains a "death domain" and has been shown to mediate g interferon-induced apoptosis. The introduction of DAP kinase into highly metastatic carcinoma clones lacking DAP kinase expression was shown to result in the suppression of metastasis, thus linking suppression of apoptosis to metastasis. ZIP kinase contains a leucine zipper domain, which is necessary for homodimerization and for interaction with other leucine zipper proteins. ZIP kinase dimerizes with ATF-4, an ATF/CREB transcription factor family member that contains a leucine zipper. Overexpression of ZIP kinase was shown to result in morphological changes associated with apoptosis in NIH/3T3 cells.
• 产品名称Anti-DAPK3 antibody [4H-C8]
• 分子量53 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus, Cytoplasm.
• 其它名称
  • DAP kinase 3 antibody
  • DAP like kinase antibody
  • DAP-like kinase antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of ZIP Kinase on human ZIP Kinase recombinant protein using anti- ZIP Kinase antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of ZIP Kinase on HEK293 (1) and ZIP Kinase -hIgGFc transfected HEK293 (2) cell lysate using anti- ZIP Kinase antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of ZIP Kinase on A431 (1) and K562 (2) cell lysate using anti- ZIP Kinase antibody at 1/1,000 dilution.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti- ZIP Kinase antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of A431 cells with ZIP Kinase antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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