Anti-BTRC antibody [D8-C5]

Anti-BTRC antibody [D8-C5]

• 概述

• 产品描述b-tranducin repeats containing protein (b-TrCP), also designated E3RSIkB or FWD1, and HOS (homologous to Slimb) are F-box proteins that function as substrate recognition subunits of ubiquitin ligases. HOS and b-TrCP differ in their amino-terminal regions, but exhibit high homology within the F-box and WD40 repeat-containing regions. b-TrCP mediates ubiquitin/proteasome-dependent degradation of CD4 and ubiquitination of various proteins including IkB and b-catenin. HOS has also been shown to regulate the degradation of IkB and b-catenin in a similar manner.
• 产品名称Anti-BTRC antibody [D8-C5]
• 分子量69 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm. Nucleus
• 其它名称
  • Beta transducin repeat containing E3 ubiquitin protein ligase antibody
  • beta TrCP1 antibody
  • beta-transducin repeat containing antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of BTRC on human BTRC recombinant protein using anti-BTRC antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of BTRC on HEK293 (1) and BTRC-hIgGFc transfected HEK293 (2) cell lysate using anti-BTRC antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of BTRC on different cell lysate using anti-BTRC antibody at 1/1,000 dilution.
  Positive control: Line1: Ramos Line2: MCF-7 Line3: K562

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-BTRC antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-BTRC antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of Hela cells with BTRC antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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