Anti-T antibody [A2-H9]

Anti-T antibody [A2-H9]

• 概述

• 产品描述The T-box gene family consists of members that share a unique DNA binding domain. The best characterized T-box (Tbx) gene, Brachyury or T, encodes a transcription factor that plays an important role in early vertebrate development. Tbx genes are a family of developmental regulators with more than 20 members recently identified among invertebrates and vertebrates. Mutations in Tbx genes have been found to cause several human diseases. The understanding of functional mechanisms of Tbx products has come mainly from the prototypical T/Brachyury protein, which is a transcription activator. The T-domain is a highly conserved DNA-binding motif originally defined in Brachyury and characteristic of the Tbx family of transcription factors. The murine Brachyury (T) gene is required in posterior mesoderm formation and axial development. Mutant embryos lacking T gene function are deficient in notochord differentiation and posterior mesoderm formation, but develop anterior mesoderm.
• 产品名称Anti-T antibody [A2-H9]
• 分子量47 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus.

• 应用

  WB: 1:500-1:1,000
  IHC-P: 1:100-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of Brachyury on human Brachyury recombinant protein using anti- Brachyury antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of Brachyury on HEK293 (1) and Brachyury -hIgGFc transfected HEK293 (2) cell lysate using anti- Brachyury antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of Brachyury on Raji (1) and Jurkat (2) cell lysate using anti- Brachyury antibody at 1/1,000 dilution.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti- Brachyury antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti- Brachyury antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of Hela cells with Brachyury antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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