Anti-CD33 antibody [B7-C6]

Anti-CD33 antibody [B7-C6]

• 概述

• 产品描述CD33 is a type I transmembrane glycoprotein that is found on granulocyte and macrophage precursors in the bone marrow, but is absent from pluripotent stem cells. CD33 is also expressed on monocytes in peripheral blood. It is used as a marker to distinguish myelogenous leukemia cells from lymphoid or erythroid leukemias. CD33 may function as a sialic acid-dependent cell adhesion molecule.
• 产品名称Anti-CD33 antibody [B7-C6]
• 分子量70 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cell membrane.
• 其它名称
  • CD 33 antibody
  • CD33 antibody
  • CD33 antigen (gp67) antibody
  more

• 应用

  WB: 1:500-1:1,000
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of CD33 on human CD33 recombinant protein using anti-CD33 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of CD33 on HEK293 (1) and CD33-hIgGFc transfected HEK293 (2) cell lysate using anti-CD33 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of CD33 on THP-1 (1) cell lysate using anti-CD33 antibody at 1/1,000 dilution.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-CD33 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human endometrium tissue using anti-CD33 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of Hela cells with CD33 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

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