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Anti-CRTC1 antibody [A2-B5]
WB: 1:500-1:1,000
ICC: 1:100-1:500
FC: 1:100-1:200
Fig1: Western blot analysis of CRTC1 on CRTC1-hIgGFc transfected HEK293 cell lysate using anti-CRTC1 antibody at 1/1,000 dilution.
Fig2: ICC staining CRTC1 (green) and actin filaments (red) in U251 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton
Fig3: ICC staining CRTC1 (green) and actin filaments (red) in NTERA2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Flow cytometric analysis of K562 cells with CRTC1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).
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