Anti-CRTC1 antibody [A2-B5]

Anti-CRTC1 antibody [A2-B5]

• 概述

• 产品描述The TORC (transducer of regulated cAMP response element-binding) proteins, TORC1 and TORC2, are potent CREB coactivators that are exported from the nucleus in a CRM1-dependent manner. The translocation of TORC proteins is a conserved step in the activation of CRE-mediated gene expression induced by cAMP. TORC1 and TORC2 operate via phosphorylation-dependent interactions.
• 产品名称Anti-CRTC1 antibody [A2-B5]
• 分子量67 kDa
• 种属反应性Human
• 验证应用WB,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm. Nucleus.
• 其它名称
  • KIAA0616 antibody
  • CREB regulated transcription coactivator 1 antibody
  • CREB-regulated transcription coactivator 1 antibody
  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:100-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of CRTC1 on CRTC1-hIgGFc transfected HEK293 cell lysate using anti-CRTC1 antibody at 1/1,000 dilution.

   

  

  Fig2: ICC staining CRTC1 (green) and actin filaments (red) in U251 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton

   

  

  Fig3: ICC staining CRTC1 (green) and actin filaments (red) in NTERA2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Flow cytometric analysis of K562 cells with CRTC1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

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