Anti-PCDH9 antibody [A2-G3]

Anti-PCDH9 antibody [A2-G3]

• 概述

• 产品描述Potential calcium-dependent cell-adhesion protein.This gene encodes a member of the protocadherin family, and cadherin superfamily, of transmembrane proteins containing cadherin domains. These proteins mediate cell adhesion in neural tissues in the presence of calcium. The encoded protein may be involved in signaling at neuronal synaptic junctions. Sharing a characteristic with other protocadherin genes, this gene has a notably large exon that encodes multiple cadherin domains and a transmembrane region. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
• 产品名称Anti-PCDH9 antibody [A2-G3]
• 分子量136 kDa
• 种属反应性Human
• 验证应用WB,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cell membrane.
  
• 其它名称
  • PCDH9 antibody
  • PCDH9_HUMAN antibody
  • Protocadherin-9 antibody

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of PCDH9 on human PCDH9 recombinant protein using anti-PCDH9 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of PCDH9 on HEK293 (1) and PCDH9-hIgGFc transfected HEK293 (2) cell lysate using anti-PCDH9 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of PCDH9 on C6 cell lysate using anti-PCDH9 antibody at 1/1,000 dilution.

   

  

  Fig4: ICC staining PCDH9 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Flow cytometric analysis of Hela cells with PCDH9 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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