Anti-MICA antibody [A4C5]

Anti-MICA antibody [A4C5]

• 概述

• 产品描述MHC class I polypeptide–related sequence A (MICA) is a highly polymorphic cell surface glycoprotein encoded by the MICA gene located within MHC locus. MICA is related to MHC class I and it has similar domain structure, however, it is not associated with β2-microglobulin nor binds peptides as conventional MHC class I molecules do. MICA rather functions as a stress-induced ligand (as a danger signal) for integral membrane protein receptor NKG2D ("natural-killer group 2, member D"). MICA is broadly recognized by NK cells, γδ T cells, and CD8+ αβ T cells which carry NKG2D receptor on their cell surface and which are activated via this interaction.
• 产品名称Anti-MICA antibody [A4C5]
• 分子量Predicted band size: 43 kDa.
• 种属反应性Human
• 验证应用WB
• 抗体类型小鼠单抗
• 免疫原Recombinant protein within human MICA aa 200-300.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein G affinity purified.
• 亚细胞定位Cell membrane, Cytoplasm, Membrane.
• 其它名称
  • HLA class I antigen antibody
  • FLJ36918 antibody
  • FLJ60820 antibody
  more

• 应用

  WB: 1:500-1:1,000

•  

  

  Fig1: Western blot analysis of MICA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: MCF-7 cell lysate
  Lane 2: Hela cell lysate

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