Anti-ABCC4 antibody [H3-A7]

Anti-ABCC4 antibody [H3-A7]

• 概述

• 产品描述The two members of the large family of ABC transporters known to confer multidrug resistance in human cancer cells are the Mdr-1 P-glycoprotein and the multidrug-resistance protein MRP1. MRP1 is an integral membrane protein that contains an MDR-like core, an N-terminal membrane-bound region and a cytoplasmic linker, and it is expressed in various cerebral cells, as well as in lung, testis and peripheral blood. The MRP gene family also includes MRP2, which is alternatively designated cMOAT (for canalicular multispecific organic anion transporter), and MRP3, which are both conjugate export pumps expressed predominantly in hepatocytes. MRP2 localizes exclusively to the apical membrane and is constitutively expressed at a high level in normal liver cells. Conversely, MRP3 localizes to the basolateral membrane where it also mediates the transport of the organic anion S-(2,4-dinitrophenyl-) glutathione toward the basolateral side of the membrane. MRP3 is normally expressed at comparatively lower levels than MRP2 and increases only when secretion across the apical membrane by MRP2 is impaired. MRP6 is highly expressed in liver and kidney, whereas MRP4 and MRP5 are detected in various tissues, yet at much lower levels of expression.

• 产品名称Anti-ABCC4 antibody [H3-A7]

• 分子量150 kDa

• 种属反应性Human

• 验证应用WB,IHC-P,FC

• 抗体类型小鼠单抗

• 免疫原Recombinant protein

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度2 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG1

• 纯化方式Protein A purified.

• 亚细胞定位Membrane.

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• ​其它名称

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  • ABCC 4 antibody

  • ABCC4 antibody

  • ATP binding cassette sub family C (CFTR/MRP) member 4 antibody

• 应用

  WB: 1:500-1:1,000
  IHC-P: 1:100-1:200
  FC: 1:100-1:200

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  Fig1: Western blot analysis of ABCC4 on human ABCC4 recombinant protein using anti-ABCC4 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of ABCC4 on HEK293 (1) and ABCC4-hIgGFc transfected HEK293 (2) cell lysate using anti-ABCC4 antibody at 1/1,000 dilution.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ABCC4 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human esophagus cancer tissue using anti-ABCC4 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of A549 cells with ABCC4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red)

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！