Anti-CHRNA6 antibody

Anti-CHRNA6 antibody

• 概述

• 产品描述Nicotinic acetylcholine receptors (nAChRs, AChRs) are protein complexes made up of protein subunits that coassemble to form an ion channel. This ion channel is gated through the binding of the neurotransmitter acetylcholine (ACh) to its ligand-binding site on the AChR. After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. CHRNA6 (cholinergic nicotinic receptor alpha polypeptide 6) is a neuronal acetylcholine receptor protein.
• 产品名称Anti-CHRNA6 antibody
• 分子量57 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cell junction. Cell membrane. Multi-pass membrane protein.
• 其它名称
  • AChR-alpha6 antibody
  • Acra6 antibody
  • Cholinergic receptor nicotinic alpha 6 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:100-1:500
  IHC-P: 1:200-1:1,000
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of CHRNA6 on human CHRNA6 recombinant protein using anti-CHRNA6 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of CHRNA6 on HEK293 (1) and CHRNA6-hIgGFc transfected HEK293 (2) cell lysate using anti-CHRNA6 antibody at 1/1,000 dilution.

   

  

  Fig3: ICC staining CHRNA6 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CHRNA6 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of SH-SY5Y cells with CHRNA6 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

   

  

  Fig6: Flow cytometric analysis of SK-N-SH cells with CHRNA6 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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