Anti-PLD2 antibody [D9-E4]

Anti-PLD2 antibody [D9-E4]

• 概述

• 产品描述The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.
• 产品名称Anti-PLD2 antibody [D9-E4]
• 分子量106 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Membrane. Peripheral membrane protein.
• 其它名称
  • Choline phosphatase 2 antibody
  • EC 3.1.4.4 antibody
  • hPLD 2 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:200

•  

  

  Fig1: Western blot analysis of PLD2 on human PLD2 recombinant protein using anti-PLD2 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of PLD2 on HEK293 (1) and PLD2-hIgGFc transfected HEK293 (2) cell lysate using anti-PLD2 antibody at 1/1,000 dilution.

   

  

  Fig3: ICC staining PLD2 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: ICC staining PLD2 (green) and Actin filaments (red) in SK-OV-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human renal tissues using anti-PLD2 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using anti-PLD2 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of Hela cells with PLD2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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