Anti-TRIM25 antibody [B12-B5]
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概述
- 产品描述TRIM25 also named EFP. EFP (estrogen-responsive finger protein) is a transcription factor, the content of which is regulated by estrogen. It has been identified as a member of the RING finger family, a family of proteins containing a Zn2+ binding domain designated the C3HC4 or RING finger. EFP also contains two B box domains and a coiled-coil region (a transactivation domain), which are characteristic of a subgroup of the RING finger family. Estrogen regulates the growth, differentiation and function of target cells in a variety of tissues; however, few genes have been shown to be directly regulated by estrogen. It has been speculated that EFP may mediate estrogen activity in a signaling cascade in which estrogen-ER binding to the estrogen responsive element (ERE) downstream of the EFP gene upregulates EFP gene expression. The EFP gene product may then activate transcription of secondary estrogen responsive genes. Additional studies indicate that the EFP promoter may be regulated by multiple elements and their interacting factors.
- 产品名称Anti-TRIM25 antibody [B12-B5]
- 分子量71 kDa
- 种属反应性Human
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型小鼠单抗
- 免疫原Recombinant protein
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度2 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG2b
- 纯化方式Protein A purified.
- 亚细胞定位Cytoplasm.
- 其它名称
- E3 ubiquitin/ISG15 ligase TRIM25 antibody
- EFP antibody
- Estrogen responsive finger protein antibody
more
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应用
WB: 1:500-1:2,000
ICC: 1:50-1:200
IHC-P: 1:200-1:500
FC: 1:100-1:200
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Fig1: Western blot analysis of TRIM25 on human TRIM25 recombinant protein using anti-TRIM25 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of TRIM25 on HEK293 (1) and TRIM25-hIgGFc transfected HEK293 (2) cell lysate using anti-TRIM25 antibody at 1/1,000 dilution.
Fig3: Western blot analysis of TRIM25 on different cell lysate using anti-TRIM25 antibody at 1/1,000 dilution.
Positive control:
Lane 1: MCF-7
Lane 2: MCF-7
Lane 3: K562
Lane 4: A549
Lane 5: MOLT4
Fig4: ICC staining TRIM25 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using anti-TRIM25 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-TRIM25 antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of Hela cells with TRIM25 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
![Anti-TRIM25 antibody [B12-B5]](images/202012/goods_img/92841_G_1608036381525.jpg)
