Anti-ERAS antibody [D10-B9]

Anti-ERAS antibody [D10-B9]

• 概述

• 产品描述Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells (By similarity). Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).
• 产品名称Anti-ERAS antibody [D10-B9]
• 分子量25 kDa
• 种属反应性Human
• 验证应用ICC,IHC-P
• 抗体类型小鼠单抗
• 免疫原Recombinant protein.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式ProA affinity purified.
• 亚细胞定位Cell membrane, Membrane.
• 数据链接SwissProt: Q7Z444 Human
  
• 其它名称
  • E ras antibody
  • E-Ras antibody
  • ecat5 antibody
  more

• 应用

  ICC:1:200
  IHC-P:1:200-1:500

•  

  

  Fig1: ICC staining ERAS in NCCIT cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with ERAS monoclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution.

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-ERAS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibodyat 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ERAS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody ( at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

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