Anti-AMPK alpha 1 antibody [B1-E10]

Anti-AMPK alpha 1 antibody [B1-E10]

• 概述

• 产品描述Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also it acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. It may regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also AMPK phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.
• 产品名称Anti-AMPK alpha 1 antibody [B1-E10]
• 分子量64 kDa
• 种属反应性Human
• 验证应用WB,ICC
• 抗体类型小鼠单抗
• 免疫原recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm, nucleus
• 其它名称
  • 5 AMP activated protein kinase alpha 1catalytic subunit antibody
  • 5 AMP activated protein kinase catalytic alpha 1 chain antibody
  • 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
  more

• 应用

  ICC: 1:100-1:200
  WB: 1:1,000-1:2,000

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  Fig1: Western blot analysis on Hela cell lysates using anti- AMPK alpha 1 mouse mAb.

   

  

  Fig2: ICC staining AMPK alpha 1 in PANC-1 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

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