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Human IL-21 Protein, Fc Tag

Human IL-21 Protein, Fc Tag
别名: IL21,Za11,Interleukin-21
储存条件: -20℃ 
Human IL-21 Protein, Fc Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-IL1-H5253-100ug 100ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

 

Human IL-21 Protein, Fc Tag

分子别名(Synonym)

IL21,Za11,Interleukin-21

表达区间及表达系统(Source)

Human IL-21, Fc Tag  is expressed from human 293 cells. It contains AA Gln 30 - Ser 162 .

Predicted N-terminus: Gln 30

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 41.9 kDa. The protein migrates as 45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human IL-21, Fc Tag (Cat. No. IL1-H5253) SDS-PAGE gel

Human IL-21, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

获取HPLC数据

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human IL-21 R, His Tag at 2 μg/mL (100 μL/well) can bind Human IL-21, Fc Tag  with a linear range of 0.2-8 ng/mL (QC tested).

Protocol

活性(Bioactivity)-Cell based assay

Biotinylated Human CELL_BASE

Human IL-21, Fc Tag  stimulates secretion of IFN-gamma by NK-92 human natural killer lymphoma cells. The EC50 for this effect is 0.94-1.34 μg/mL (Routinely tested).

 

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