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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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1,5-脱水葡萄糖醇(1,5-AG)ELISA Kit
| Traditional ELISA Kit | Ready-to-Use ELISA KIT | |
| Product name: | General 1,5-Anhydroglucitol (1,5-AG) ELISA Kit | |
| Method: | Competition | |
| Synonyms: |
LycoMark; 1-Deoxy-D-glucose; 1-Deoxy-D-glucopyranose; 1,5-Anhydro-D-glucitol; 1,5-Anhydrosorbitol; Aceritol; Polygalytol |
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| Detection range: | 1.56-100ug/mL | |
| Reactivity: | 1,5-AG | |
| Size: | 96T/48T | |
| Quality guarantee period: | for 12 months | |
| Catalog number: | DL-1,5-AG-Ge(traditional) | DLR-1,5-AG-Ge (ready-to-use) |
| Assay length | 1-2.5Hours | 1-2.5Hours |
| Advantages: |
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| Item | Standard | Test | |
| Description |
The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of 1,5-AG in serum, plasma, tissue homogenates and other biological fluids. |
Conform | |
| Identification | Colorimetric | Positive | |
| Composition | Traditional ELISA Kit | Ready-to-Use ELISA KIT | Conform |
| Pre-coated, ready to use 96-well strip plate 1 | Pre-coated, ready to use 96-well strip plate 1 | ||
| Plate sealer for 96 wells 2 | Plate sealer for 96 wells 2 | ||
| Standard 2 | Standard 2 | ||
| Diluents buffer 1×45mL | Standard Diluent 1×20mL | ||
| Detection Reagent A 1×120μL | Detection Solution A 1×12mL | ||
| Detection Reagent B 1×120μL | Detection Solution B 1×12mL | ||
| TMB Substrate 1×9mL | TMB Substrate 1×9mL | ||
| Stop Solution 1×6mL | Stop Solution 1×6mL | ||
| Wash Buffer (30 × concentrate) 1×20mL | Wash Buffer (30 × concentrate) 1×20mL | ||
| Instruction manual 1 | Instruction manual 1 | ||
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 1,5-AG has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 1,5-AG and unlabeled 1,5-AG (Standards or samples) with the pre-coated antibody specific to 1,5-AG. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 1,5-AG in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 1,5-AG in the sample.
Matrices listed below were spiked with certain level of recombinant 1,5-AG and the recovery rates were calculated by comparing the measured value to the expected amount of 1,5-AG in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 80-102 | 91 |
| EDTA plasma(n=5) | 81-100 | 90 |
| heparin plasma(n=5) | 80-89 | 84 |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 1,5-AG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1：2 | 1：4 | 1：8 | 1：16 |
| serum(n=5) | 82-96% | 83-98% | 81-99% | 93-101% |
| EDTA plasma(n=5) | 88-101% | 86-95% | 90-102% | 80-93% |
| heparin plasma(n=5) | 80-91% | 82-90% | 95-104% | 79-95% |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 1,5-AG were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 1,5-AG were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
Note:
To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37℃;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
