Anti-Neuron-specific class Ⅲ β-tubulin antibody

Anti-Neuron-specific class Ⅲ β-tubulin antibody

• 概述

• 产品描述Tubulin is a compound of subunits of A tubulin and B tubulin. Class III beta tubulin (beta III-tubulin) is a vertebrate tubulin isotype specific to the neurons and mammalian testis cells, making it an ideal neuronal marker. Overexpression of class III beta tubulin is associated with the resistances of microtubule-targeted cancer drugs in lung cancer cell lines, breast cancer cell lines, and ovarian tumors.
• 产品名称Anti-Neuron-specific class Ⅲ β-tubulin antibody
• 分子量50 kDa
• 种属反应性Human, Mouse, Rat
• 验证应用WB, ICC, IHC-P, FC
• 抗体类型兔多抗
• 免疫原This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of N-terminal residues of tubulin beta-3.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG
• 纯化方式Peptide affinity purified.
• 亚细胞定位Cytoplasm. Cytoskeleton. Microtubule.
• 数据链接Q13509 Human
  Q9ERD7 Mouse
  Q4QRB4 Rat
  
• 其它名称beta 3 tubulin antibody
  beta-4 antibody
  CDCBM antibody
  CDCBM1 antibody
  CFEOM3 antibody
  CFEOM3A antibody
  FEOM3 antibody
  M(beta)3 antibody
  M(beta)6 antibody
  MC1R antibody
  Neuron specific beta III Tubulin antibody
  Neuron-specific class III beta-tubulin antibody
  QccE-11995 antibody
  QccE-15186 antibody
  TBB3_HUMAN antibody
  Tubb 3 antibody
  TUBB3 antibody
  TUBB4 antibody
  Tubulin beta 3 antibody
  Tubulin beta 3 chain antibody
  Tubulin beta 4 antibody
  Tubulin beta III antibody
  Tubulin beta-3 chain antibody
  Tubulin beta-4 chain antibody
  Tubulin beta-III antibody
  more

• 应用

  WB: 1:5,000-1:10,000
  ICC: 1:50-1:200
  IHC-P: 1:100-1:400
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of Tubulin beta-3 chain on Hela cell lysate using anti-Tubulin beta-3 chain antibody at 1/10,000 dilution.

   

  

  Fig2: ICC staining Tubulin beta-3 chain in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining Tubulin beta-3 chain in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Tubulin beta-3 chain antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Tubulin beta-3 chain antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Tubulin beta-3 chain antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of Hela cells with Tubulin beta-3 chain antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as

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