Anti-XIAP/BIRC4 antibody
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概述
- 产品描述Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B.
- 产品名称Anti-XIAP/BIRC4 antibody
- 分子量55 KDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,FC
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human XIAP 201-330/496
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cytoplasm.
- 其它名称X-linked Inhibitor of Apoptosis Protein
RIAP-3
Baculoviral IAP repeat-containing protein 4
E3 ubiquitin-protein ligase XIAP
Inhibitor of apoptosis protein 3
X-linked IAP
IAP-like protein
HILP
BIRC4
HILP
XLP2
ILP1
Xiap
MIHA
hILP
IAP3
API3
XIAP
XIAP_HUMAN
ILP
hILP
IAP-3
hIAP-3
hIAP3
X-linked inhibitor of apoptosis protein.
more
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应用
WB:1:500-2000
FC:3ug/Test
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Fig1: Blank control (Black line): HUVEC(Black).
Primary Antibody (green line): Rabbit Anti-XIAP antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Fig2: Sample:Liver(Mouse) Lysate at 40 ug
Primary: Anti-XIAP/BIRC4at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 53 kD
Fig3: Sample:
Lymph node(Mouse) Lysate at 40 ug
Bone(Mouse) Lysate at 40 ug
Primary: Anti-XIAP/BIRC4 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 53 kD
Fig4: Sample:Skin(Mouse) Lysate at 40 ug
Primary: Anti-XIAP/BIRC4 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 53 kD
Fig5: Sample: Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-XIAP/BIRC4 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
