Anti-ESD antibody [J0-8]

Anti-ESD antibody [J0-8]

• 概述

• 产品描述ESD (esterase D) is also known as S-formylglutathione hydrolase and is a 282 amino acid protein that is a member of the esterase D family. ESD is highly expressed in placenta, kidney, liver and erythrocytes, and is localized to the cytoplasm, as well as to cytoplasmic vesicles. The main function of ESD is to detoxify formaldehyde while providing energy. Formaldehyde is oxidized by ADH5 which yields S-formylglutathione. ESD then catalyzes the hydrolysis of S-formylglutathione to the reduced forms of formic acid and glutathione. In addition, ESD hydrolyzes a variety of different neutral ester substrates and can act as a carboxylesterase. ESD may also act as a cysteine hydrolase which is inactivated by thiol alkylating agents. ESD gene polymorphism can lead to reduced enzymatic activity which may cause susceptibility to many conditions, including toxic liver cirrhosis, retinoblastoma, obesity and autism.
• 产品名称Anti-ESD antibody [J0-8]
• 分子量31 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein within Human ESD of aa 1-300.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2c
• 纯化方式Protein affinity purified.
• 亚细胞定位Cytoplasm. Cytoplasmic vesicle.
• 其它名称
  • EC 3.1.2.12 antibody
  • Es-10 antibody
  • Es10 antibody
  more

• 应用

  WB:1:1,000-1:2,000
  ICC:1:100
  IHC-P:1:100
  FC:1:50-1:100

•  

  

  Fig1: Western blot analysis of ESD on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:2,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: K562 cell lysate, untreated
  Lane 2: Jurkat cell lysate, untreated

   

  

  Fig2: ICC staining ESD in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with ESD monoclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

   

  

  Fig3: ICC staining ESD in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with ESD monoclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

   

  

  Fig4: ICC staining ESD in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with ESD monoclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ESD antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibod at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ESD antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

   

  

  Fig7: Flow cytometric analysis of ESD was done on Jurkat cells. The cells were fixed, permeabilized and stained with ESD antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After in

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