Anti-Frizzled-5 antibody
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概述
- 产品描述Frizzled-5 belongs to the G-protein coupled receptor Fz/Smo family, contains 585 amino acids. It is localized at the plasma membrane and also found at the Golgi. Fzds have a large extracellular N-terminal region containing a cysteine-rich domain (CRD), which is involved in binding to Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes.
- 产品名称Anti-Frizzled-5 antibody
- 分子量64 kDa
- 种属反应性Human
- 验证应用WB,ICC,FC
- 抗体类型兔多抗
- 免疫原Synthetic peptide within Human Frizzled-5 of aa 168-181.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Cell membrane.Golgi apparatus. Membrane. Synapse.
- 其它名称C2orf31 antibody
DKFZP434E2135 antibody
Frizzled 5 seven transmembrane spanning receptor antibody
Frizzled class receptor 5 antibody
Frizzled family receptor 5 antibody
Frizzled homolog 5 antibody
Frizzled-5 antibody
Frizzled5 antibody
Fz 5 antibody
Fz-5 antibody
Fz5 antibody
FZD 5 antibody
fzd5 antibody
FZD5_HUMAN antibody
FzE 5 antibody
FzE5 antibody
HFZ 5 antibody
hFz5 antibody
MGC129692 antibody
Seven transmembrane receptor frizzled 5 antibody
Uncharacterized protein C2orf31 antibody
Wnt receptor antibody
more
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应用
WB:1:500-1:1,000
ICC:1:50-1:200
FC:1:50-1:100
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Fig1: Western blot analysis of Frizzled-5 on human ES cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: ICC staining Frizzled-5 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibodyat a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Fig3: ICC staining Frizzled-5 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibodat a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Fig4: Flow cytometric analysis of Frizzled-5 was done on HepG2 cells. The cells were fixed, permeabilized and stained with Frizzled-5 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution.
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