Anti-IL-1R2 antibody

Anti-IL-1R2 antibody

• 概述

• 产品描述Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.
• 产品名称Anti-IL-1R2 antibody
• 分子量44 KDa
• 种属反应性Human,Mouse,Rat,Dog
• 验证应用WB,IHC-P,FC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human IL-1R II 301-398/398
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Isoform Short: Secreted. Isoform Long: Cell membrane; Single-pass type I membrane protein.
• 其它名称IL1 Receptor II
  IL-1R II
  IL1 Receptor 2
  interleukin-1 receptor type 2 precursor
  IL1R2
  CD121b
  IL1RB
  CD121
  CD121 antigen-like family member B
  CD121b
  CD121B Antigen
  CDW121B
  IL-1R-2
  IL-1R-beta
  IL-1R2
  IL-1RT-2
  IL-1RT2
  IL1R Beta
  Il1r-2
  IL1R2
  IL1RB
  Interleukin 1 receptor, beta, type II
  Interleukin 1 receptor, type II
  Interleukin-1 receptor beta
  Interleukin-1 receptor type 2
  MGC137383
  MGC47725
  p68
  IL1 Receptor II
  IL1R2_HUMAN.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:1μg/test

•  

  

  Fig1: Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL-1R2) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig2: Blank control: RSC96 cells(blue).
  Primary Antibody: Rabbit Anti-IL-1R2 antibody Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

   

  

  Fig3: Sample: SW480 Cell (Human) Lysate at 40 ug
  Primary: Anti- IL-1R2at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 44 kD
  Observed band size: 44 kD

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