Anti-Inhibin beta B antibody
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概述
- 产品描述Inhibin, beta B, also known as INHBB, is a protein which in humans is encoded by the INHBB gene. INHBB is a subunit of both activin and inhibin, two closely related glycoproteins with opposing biological effects. Inhibins are heterodimeric glycoproteins composed of an α subunit (INHA) and one of two homologous, but distinct, β subunits (βA or βB, this protein). mRNA for the two subunits has been demonstrated in the testes of adult rats. Inhibin can bind specifically to testicular interstitial cells throughout development and may be an important regulator of Leydig cell testosterone production or interstitial cell function. The inhibin beta B subunit joins the α subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumour-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone.
- 产品名称Anti-Inhibin beta B antibody
- 分子量13/45 KDa
- 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Sheep,Guinea Pig
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human Inhibin beta B 251-350/407
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式Affinity purified by Protein A.
- 亚细胞定位Secreted.
- 其它名称MGC157939
Activin A
Activin AB alpha polypeptide
Activin AB
Activin AB beta polypeptide
Activin B
Activin beta A chain
Activin beta B chain
ActivinA
ActivinAB
ActivinB
EDF
Erythroid differentiation protein
follicle stimulating hormone releasing protein
FRP
FSH releasing factor
FSH releasing protein
INHBA
INHBB
Inhibin
Inhibin beta 1
Inhibin beta 2
Inhibin beta A
Inhibin beta A chain
Inhibin beta A subunit
Inhibin beta B
Inhibin beta B chain
Inhibin beta B subunit
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Sample:
A431(Human) Cell Lysate at 40 ug
Primary: Anti- Inhibin beta B at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 50 kD
Fig2: Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti- Inhibin beta B at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 50 kD
Fig3: Paraformaldehyde-fixed, paraffin embedded (rat ovary tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Inhibin beta B) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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