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Anti-Nfic antibody
WB: 1:500
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
Fig1: Western blot analysis of Nfic on different cell lysate using anti-Nfic antibody at 1/500 dilution.
Positive control:
Lane 1: A549
Lane 2: SiHa
Fig2: ICC staining Nfic in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Nfic in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Nfic in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Nfic antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Fig6: Flow cytometric analysis of SiHa cells with Nfic antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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