Anti-Calnexin antibody
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概述
- 产品描述Calnexin and Calregulin (also called calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum, Calnexin to the membrane and Calregulin to the lumen. Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum. Calregulin has both low- and high-affinity calcium-binding sites. Neither Calnexin nor Calregulin contains the calcium-binding “E-F hand” motif found in calmodulins. Calnexin and Calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins.
- 产品名称Anti-Calnexin antibody
- 分子量90 kDa
- 种属反应性Human,Mouse
- 验证应用WB,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Synthetic peptide within C-terminal residues of rat Calnexin.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Endoplasmic reticulum.
- 其它名称Calnexin antibody
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CANX antibody
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IP90 antibody
Major histocompatibility complex class I antigen-binding protein p88 antibody
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more
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应用
WB: 1:500-1:2,000
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of Calnexin on mouse lung tissue (1) and Daudi cell (2) lysate using anti-Calnexin antibody at 1/500 dilution.
Fig2: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Calnexin antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Calnexin antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Calnexin antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calnexin antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of SKOV-3 cells with Calnexin antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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