Anti-GAPDH antibody
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概述
- 产品描述GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
- 产品名称Anti-GAPDH antibody
- 分子量36 kDa
- 种属反应性Human,Mouse,Rat,Zebrafish,Rabbit
- 验证应用WB, ICC
- 抗体类型小鼠单抗
- 免疫原Synthetic peptide within human GAPDH aa 10-50.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度2 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgM
- 纯化方式Protein L purified.
- 亚细胞定位Cytoplasm
- 其它名称
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应用
WB: 1:5,000-1:10,000
ICC: 1:200-1:500
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Fig1: Western blot analysis of GAPDH on different cell lysates using anti-GAPDH antibody at 1/5000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Hela
Lane 3: PC12
Lane 4: NIH/3T3
Lane 5: MCF-7
Lane 6: Rabbit liver
Lane 7:Zebrafish
Fig2: ICC staining GAPDH in F9 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining GAPDH in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining GAPDH in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Western blot analysis of GAPDH on hybrid fish (crucian-carp) liver tissue lysate using anti-GAPDH antibody at 1/500 dilution.
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