Anti-CDKN2A/p16INK4a antibody
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概述
- 产品描述This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.
- 产品名称Anti-CDKN2A/p16INK4a antibody
- 分子量17 kDa
- 种属反应性Human
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Synthetic peptide within C-terminal human CDKN2A/p16INK4a.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Nucleus. Cytoplasm.
- 其它名称CCM2 antibody
CDK4 inhibitor p16 INK4 antibody
CDK4I antibody
CDKN2 antibody
CDKN2A antibody
Cell cycle negative regulator beta antibody
CMM2 antibody
Cyclin dependent kinase 4 inhibitor A antibody
Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody
Cyclin Dependent Kinase Inhibitor 2A antibody
Cyclin dependent kinase inhibitor 2A isoform 4 antibody
Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody
Cyclin dependent kinase inhibitor p16 antibody
INK4 antibody
INK4A antibody
MLM antibody
MTS1 antibody
Multiple tumor suppressor 1 antibody
p14 antibody
p16 antibody
P16INK4 antibody
p16INK4a antibody
p19 antibody
p19Arf antibody
TP16 antibody
more
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应用
WB: 1:500
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of CDKN2A/p16INK4a on SiHa cell lysate using anti-CDKN2A/p16INK4a antibody at 1/500 dilution.
Positive control:
Lane 1: SiHa cell lysate
Lane 2: SiHa cell lysate with immunization peptide
Fig2: ICC staining CDKN2A/p16INK4a in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining CDKN2A/p16INK4a in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining CDKN2A/p16INK4a in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CDKN2A/p16INK4a antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Fig6: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-CDKN2A/p16INK4a antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Fig7: Flow cytometric analysis of 293T cells with CDKN2A/p16INK4a antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the
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