Anti-PRSS10 antibody

Anti-PRSS10 antibody

• 概述

• 产品描述Plasma membrane-anchored serine protease that participates in proteolytic cascades of relevance for the normal physiologic function of the prostate . Androgen-induced TMPRSS2 activates several substrates that include pro-hepatocyte growth factor/HGF, the protease activated receptor-2/F2RL1 or matriptase/ST14 leading to extracellular matrix disruption and metastasis of prostate cancer cells . In addition, activates trigeminal neurons and contribute to both spontaneous pain and mechanical allodynia (By similarity).By similarity3 Publications (Microbial infection) Facilitates human coronaviruses SARS-CoV and SARS-CoV-2 infections via two independent mechanisms, proteolytic cleavage of ACE2 receptor which promotes viral uptake, and cleavage of coronavirus spike glycoproteins which activates the glycoprotein for host cell entry . Proteolytically cleaves and activates the spike glycoproteins of human coronavirus 229E (HCoV-229E) and human coronavirus EMC (HCoV-EMC) and the fusion glycoproteins F0 of Sendai virus (SeV), human metapneumovirus (HMPV), human parainfluenza 1, 2, 3, 4a and 4b viruses (HPIV). Essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9); involved in proteolytic cleavage and activation of hemagglutinin (HA) protein which is essential for viral infectivity.
• 产品名称Anti-PRSS10 antibody
• 分子量54 KDa
• 种属反应性Human, Mouse, Rat, Pig, Cow, Sheep
• 验证应用WB, IHC-P,ICC,IF
• 抗体类型兔多抗
• 免疫原Synthetic peptide within human PRSS10 aa 301-400.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cell membrane, Membrane, Secreted.
• 其它名称Epitheliasin
  PRSS10
  Serine protease 10
  TMPRSS2
  TMPRSS2 ERG FUSION GENE, INCLUDED
  TMPRSS2 ETV1 FUSION GENE, INCLUDED
  TMPS2_HUMAN
  Transmembrane protease serine 2 catalytic chain
  Transmembrane protease, serine 2
  Transmembrane protease, serine 2, EC 3.4.219
  Transmembrane protease, serine 2, EC 3.4.219
  D16Ertd61e.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  ICC:1:50-1:200
  IF:1:50-1:200

•  

  

  Fig1: Western blot analysis of PRSS10 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: Du145 cell lysate
  Lane 2: Mouse placenta tissue lysate

   

  

  Fig2: Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-PRSS10 Polyclonal Antibody, Unconjugated1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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