Anti-BLOS1 antibody
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概述
- 产品描述Component of the biogenesis of lysosome-related organelles complex-1 (BLOC-1), a complex that mediates the vacuolar degradative transport via the intracellular vesicle trafficking from the endosome to the vacuole. Probably regulates the PIN1 and PIN2 homeostasis through its interaction with SNX1. An eight-subunit protein complex, BLOC-1, which is involved in endosomal transport from the endosomes to the lysosomes, has been identified in both human and mice. In this study, two homologous subunits of this complex, BLOS1 (or AtGCN5L1) and BLOS2, have been characterized in Arabidopsis. Both BLOS1 and BLOS2 interacted with SNX1 on the sorting endosomes. Inducible RNAi lines with reduced levels of BLOS1 had longer primary roots and more lateral roots. Consistently, PIN1 and PIN2 were increased in BLOS1 RNAi lines, implicating an impaired transport from the endosomes to the vacuoles. These results suggest that a putative BLOC-1 complex in Arabidopsis might mediate the vacuolar degradative transport through direct interaction with SNX1 to regulate the homeostasis of PIN1 and PIN2, which is important for plant growth and development.
- 产品名称Anti-BLOS1 antibody
- 分子量17 kDa
- 种属反应性Rice,Arabidopsis thaliana
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原Synthetic peptide within thaliana BLOS1 aa 31-70.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Endosome, cytoplasm.
- 其它名称Biogenesis of lysosome-related organelles complex 1 subunit 1 antibody
BL1S1_HUMAN antibody
BLOC-1 subunit 1 antibody
Bloc1s1 antibody
BLOS1 antibody
GCN5-like protein 1 antibody
GCN5L1 antibody
Protein RT14 antibody
RT14 antibody
more
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应用
WB:1:500-1:2,000
IHC-P:1:50-1:200
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Fig1: Western blot analysis of BLOS1 on rice tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibodywas used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: Immunohistochemical analysis of paraffin-embedded A. thaliana tissue using anti-BLOS1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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