Anti-CD4 antibody

Anti-CD4 antibody

• 概述

• 产品描述Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
• 产品名称Anti-CD4 antibody
• 分子量51 kDa
• 种属反应性Human
• 验证应用WB,FC
• 抗体类型兔多抗
• 免疫原Recombinant protein with Human CD4 1-300 aa.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG
• 纯化方式Protein affinity purified.
• 亚细胞定位Cell Membrane.
• 其它名称CD 4 antibody
  CD4 (L3T4) antibody
  CD4 antibody
  CD4 antigen (p55) antibody
  CD4 antigen antibody
  CD4 molecule antibody
  CD4 receptor antibody
  CD4+ Lymphocyte deficiency, included antibody
  CD4_HUMAN antibody
  CD4mut antibody
  L3T4 antibody
  Leu3 antibody
  Ly-4 antibody
  Lymphocyte antigen CD4 antibody
  MGC165891 antibody
  OTTHUMP00000238897 antibody
  p55 antibody
  T cell antigen T4 antibody
  T cell antigen T4/LEU3 antibody
  T cell differentiation antigen L3T4 antibody
  T cell OKT4 deficiency, included antibody
  T cell surface antigen T4/Leu 3 antibody
  T cell surface antigen T4/Leu3 antibody
  T cell surface glycoprotein CD4 antibody
  T-cell surface antigen T4/Leu-3 antibody
  T-cell surface glycoprotein CD4 antibody
  W3/25 antibody
  W3/25 antigen antibody
  more

• 应用

  WB:1:500
  FC:1:50-1:100

•  

  

  Fig1: Western blot analysis of CD4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: U937 cell lysate
  Lane 2: Human thymus tissue lysate

   

  

  Fig2: Flow cytometric analysis of CD4 was done on Hela cells. The cells were fixed, permeabilized and stained with CD4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.

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