Anti-C1orf96 antibody

Anti-C1orf96 antibody

• 概述

• 产品描述May play a role in embryonic development. May be required for proper cilia beating.
• 产品名称Anti-C1orf96 antibody
• 分子量30 KDa
• 种属反应性Human,Mouse,Rat,Chicken,Dog,Horse,Rabbit
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human C1orf96 171-270/270
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cytoplasm, cytoskeleton, centrosome, centriole. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, cilium axoneme. Cell projection, axon. Cell projection, cilium. Note=Localizes
• 其它名称C1orf96
  FLJ37296
  FLJ41471
  Uncharacterized protein C1orf96
  CCSAP_HUMAN
  Centriole, cilia and spindle-associated protein
  CCSAP
  CSAP
  RP4-803J11.3.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (C1orf96) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig2: Sample: Placenta (Mouse) Lysate at 40 ug
  Primary: Anti-C1orf96 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 30kD
  Observed band size: 34kD

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