Anti-OCT-4 antibody

Anti-OCT-4 antibody

• 概述

• 产品描述Oct-4 (POU5F1) is transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). It forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Oct-4 is critical for early embryogenesis and for embryonic stem cell pluripotency. It is expressed in developing brain and highest levels also found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum.
• 产品名称Anti-OCT-4 antibody [A3-C11]
• 分子量39 kDa
• 种属反应性Human,Mouse
• 验证应用WB,ICC
• 抗体类型小鼠单抗
• 免疫原peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus
• 其它名称
  • Octamer binding transcription factor 4 antibody
  • MGC22487 antibody
  • Oct 3 antibody
  more

• 应用

  WB: 1:1,000-1:5,000
  ICC: 1:200

•  

  

  Fig1: Western blot analysis of OCT-4 on different cell lysates using anti- OCT-4 antibody at 1/1000 dilution.
  Positive control: Line 1: NCCIT Line2 :D3

   

  

  Fig2: ICC staining of OCT-4 in NCCIT cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining of OCT-4 in F9 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

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