Anti-Sodium hydrogen exchanger 1 antibody
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概述
- 产品描述The sodium-hydrogen antiporter 1 (NHE-1) also known as sodium/hydrogen exchanger 1 or SLC9A1 (SoLute Carrier family 9A1) is an isoform of sodium–hydrogen antiporter that in humans is encoded by the SLC9A1 gene. The Na+/H+ antiporter (SLC9A1) is a ubiquitous membrane-bound enzyme involved in volume- and pH-regulation of vertebrate cells. It is inhibited by the non-specific diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, tumor promoters, and others. Sodium–hydrogen antiporter 1 has been shown to interact with carbonic anhydrase II and CHP. It is also the target of the experimental drug rimeporide, which is being developed for the treatment of Duchenne muscular dystrophy.
- 产品名称Anti-Sodium hydrogen exchanger 1 antibody
- 分子量91 KDa
- 种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human NHE1 470-550/815
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式Affinity purified by Protein A.
- 亚细胞定位Membrane; Multi-pass membrane protein.
- 其它名称amiloride-sensitive
APNH
APNH1
FLJ42224
Na Li countertransporter
Na(+)/H(+) antiporter
Na(+)/H(+) exchanger 1
Na+ H+ antiporter amiloride-sensitive
Na+ H+ antiporter
Na+ H+ exchanger 1
NHE-1
NHE1
OTTHUMP00000004468
SL9A1_HUMAN
SLC9A1
Sodium hydrogen exchanger 1
Sodium/hydrogen exchanger 1
solute carrier family 9
Solute carrier family 9 member 1
Solute carrier family 9 sodium hydrogen exchanger isoform 1 antiporter Na+ H+ amiloride sensitive
Solute carrier family 9 subfamily A (NHE1 cation proton antiporter 1) member 1
Solute carrier family 9 subfamily A member 1.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Sample:
Brain(Rat) lysate at 30ug;
Liver(Rat) lysate at 30ug;
Primary: Anti-NHE1at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution;
Predicted band size : 91kD
Observed band size : 91kD
We are unsure as to the identity of these extra bands
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Sodium hydrogen exchanger 1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig3: Sample:
molt-4(human)cell Lysate at 40 ug
Primary: Anti- Sodium hydrogen exchanger 1 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 91kD
Observed band size: 91 kD
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