Anti-β-tubulin antibody

Anti-β-tubulin antibody

• 概述

• 产品描述Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. Beta-Tubulin may have bound GTP or GDP. Under certain conditions β-tubulin can hydrolyze its bound GTP to GDP plus Pi, release the Pi, and exchange the GDP for GTP.
• 产品名称Anti-β-tubulin antibody 
• 分子量50 kDa
• 种属反应性Human,Mouse,Rat,Zebrafish
• 验证应用WB, ICC, IHC-P, FC
• 抗体类型小鼠单抗
• 免疫原peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm ,cytoskeleton
• 其它名称
  • Beta 4 tubulin antibody
  • Beta 5 tubulin antibody
  • BetaTubulin antibody
  more

• 应用

  WB: 1:5,000-1:10,000
  ICC: 1:200
  IHC-P: 1:20
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of β-tubulin on different cell lysates using anti-β-tubulin antibody at 1/5000 dilution.
  Positive control:
  Lane 1: NCCIT
  Lane 2: NIH/3T3
  Lane 3: PC12
  Lane 4: Mouse heart
  Lane 5: F9
  Lane 6: zebrafish brain
  Lane 7: Hela

   

  

  Fig2: ICC staining β-tubulin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC stainingβ-tubulin in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: ICC stainingβ-tubulin in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-β-tubulin antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of HeLa cells with β-tubulin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.

   

  

  Fig7: Western blot analysis of β-tubulin on hybrid fish (crucian-carp) brain tissue lysate using anti-β-tubulin antibody at 1/500 dilution.

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