Anti-Rab6b antibody [E4-D12]

Anti-Rab6b antibody [E4-D12]

• 概述

• 产品描述Seems to have a role in retrograde membrane traffic at the level of the Golgi complex. May function in retrograde transport in neuronal cells. RAB6B (RAB6B, Member RAS Oncogene Family) is a Protein Coding gene. Among its related pathways are Sertoli-Sertoli Cell Junction Dynamics. GO annotations related to this gene include GTP binding and GTPase activity. An important paralog of this gene is RAB41
• 产品名称Anti-Rab6b antibody [E4-D12]
• 分子量23 kDa
• 种属反应性Human,Rat
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Golgi apparatus membrane. Cytoplasmic vesicle
• 其它名称
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  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:200

•  

  

  Fig1: Western blot analysis of Rab6b on human Rab6b recombinant protein using anti-Rab6b antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of Rab6b on HEK293 (1) and Rab6b-hIgGFc transfected HEK293 (2) cell lysate using anti-Rab6b antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of Rab6b on different cell lysate using anti-Rab6b antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: C6
  Lane 2: HT-29
  Lane 3: PC-12

   

  

  Fig4: ICC staining Rab6b (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: ICC staining Rab6b (green) and Actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Rab6b antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-Rab6b antibody. Counter stained with hematoxylin.

   

  

  Fig8: Flow cytometric analysis of Hela cells with Rab6b antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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