Anti-ATF6 antibody
-
概述
- 产品描述Transmembrane glycoprotein of the endoplasmic reticulum that functions as a transcription activator and initiates the unfolded protein response (UPR) during endoplasmic reticulum stress. Cleaved upon ER stress, the N-terminal processed cyclic AMP-dependent transcription factor ATF-6 alpha translocates to the nucleus where it activates transcription of genes involved in the UPR. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N9-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina.
- 产品名称Anti-ATF6 antibody
- 分子量75 kDa
- 种属反应性Human,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein within full sequence of human ATF6.
- 偶联Non-conjugated
-
性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze/thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein A purified.
- 亚细胞定位Endoplasmic reticulum membrane. Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. THBS4 promotes its nuclear shuttling.
- 其它名称Activating transcription factor 6 alpha antibody
Activating transcription factor 6 antibody
ATF 6 antibody
ATF6 alpha antibody
ATF6 antibody
ATF6-alpha antibody
ATF6A antibody
ATF6A_HUMAN antibody
cAMP dependent transcription factor ATF 6 alpha antibody
cAMP-dependent transcription factor ATF-6 alpha antibody
Cyclic AMP dependent transcription factor ATF 6 alpha antibody
DKFZp686P2194 antibody
ESTM49 antibody
FLJ21663 antibody
Processed cyclic AMP dependent transcription factor ATF 6 alpha antibody
Processed cyclic AMP-dependent transcription factor ATF-6 alpha antibody
more
-
应用
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
-
Fig1: Western blot analysis of ATF6 on Hela cell lysate using anti-ATF6 antibody at 1/500 dilution.
Fig2: ICC staining ATF6 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining ATF6 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ATF6 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ATF6 antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of K562 cells with Thioredoxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
