Anti-GRP78 antibody
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概述
- 产品描述Plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate (By similarity). GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation.
- 产品名称Anti-GRP78 antibody
- 分子量72 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Peptide.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Endoplasmic reticulum. Cytoplasm.
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应用
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of GRP78 on different lysates using anti-GRP78 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse liver tissue
Lane 2: Rat liver tissue
Lane 3: HepG2
Fig2: ICC staining GRP78 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining GRP78 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining GRP78 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GRP78 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GRP78 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-GRP78 antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-GRP78 antibody. Counter stained with hematoxylin.
Fig9: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-GRP78 antibody. Counter stained with hematoxylin.
Fig10: Flow cytometric analysis of Jurkat cells with GRP78 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the second
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