Anti-BMAL1 antibody
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概述
- 产品描述BMAL1, ARNTL2 (aryl hydrocarbon receptor nuclear translocator-like 2) is a basic Helix-Loop-Helix-Per-Arnt-Sim (bHLH-PAS) transcription factor. ARNTL2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription. Also, in umbilical vein endothelial cells, ARNTL2 activates SERPINE1 through E-box sites. This transactivation is inhibited by PER2 and CRY1. The NPAS2-ARNTL/BMAL1 heterodimer positively regulates the expression of MAOA, F7 and LDHA and modulates the circadian rhythm of daytime contrast sensitivity by regulating the rhythmic expression of adenylate cyclase type 1 (ADCY1) in the retina. The CLOCK-ARNTL/BMAL1 heterodimer also recognizes the non-canonical E-box motifs 5'-AACGTGA-3' and 5'-CATGTGA-3'. Essential for the rhythmic interaction of CLOCK with ASS1 and plays a critical role in positively regulating CLOCK-mediated acetylation of ASS1.
- 产品名称Anti-BMAL1 antibody
- 分子量68 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Cytoplasm, Nucleus.
- 其它名称ARNT like protein 1 brain and muscle antibody
Arntl antibody
Aryl hydrocarbon receptor nuclear translocator like antibody
Aryl hydrocarbon receptor nuclear translocator like protein 1 antibody
Aryl hydrocarbon receptor nuclear translocator-like protein 1 antibody
Basic helix loop helix PAS orphan MOP3 antibody
Basic helix loop helix PAS protein MOP3 antibody
Basic-helix-loop-helix-PAS protein MOP3 antibody
bHLH PAS protein JAP3 antibody
bHLH-PAS protein JAP3 antibody
bHLHe5 antibody
BMAL 1 antibody
BMAL1_HUMAN antibody
BMAL1c antibody
Brain and muscle ARNT like 1 antibody
Brain and muscle ARNT-like 1 antibody
CG8727 PA antibody
Class E basic helix-loop-helix protein 5 antibody
cycle antibody
JAP 3 antibody
JAP3 antibody
Member of PAS protein 3 antibody
Member of PAS superfamily 3 antibody
MGC47515 antibody
MOP 3 antibody
MOP3 antibody
PAS domain-containing protein 3 antibody
PASD 3 antibody
PASD3 antibody
TIC antibody
more
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应用
WB: 1:500-2,000
ICC: 1:100-1:500
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of BMAL1 on SYSH5Y cell lysates using anti-BMAL1 antibody at 1/1,000 dilution.
Fig2: ICC staining BMAL1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining BMAL1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cncer tissue using anti-BMAL1 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-BMAL1 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-BMAL1 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-BMAL1 antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-BMAL1 antibody. Counter stained with hematoxylin.
Fig9: Flow cytometric analysis of SHSY5Y cells with BMAL1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the seconda
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