Anti-AMACR antibody
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概述
- 产品描述P504S, also known as AMACR (α-methylacyl-CoA racemase), 2-methylacyl-CoA racemase or RACE, is an enzyme belonging to the caiB/baiF CoA-transferase family. Localizing to the peroxisome and mitochondrion, P504S plays a role in the metabolism of branched-chain fatty acids and bile acid intermediates. More specifically, P504S catalyzes the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers which can then be degraded by peroxisomal β-oxidation. Mutations in the gene encoding P504S can lead to AMACR deficiency, a disease characterized by increased concentrations of pristanic acid that is associated with adult onset sensory motor neuropathy, and/or CBAS4 (congenital bile acid synthesis defect type 4), a disorder characterized by intrahepatic cholestasis, absence of cholic acid from bile, neonatal jaundice and bile duct deficiency. In addition, P504S is overexpressed in prostate cancer and is believed to be functionally important for prostate cancer cell growth.
- 产品名称Anti-AMACR antibody
- 分子量42 kDa
- 种属反应性Human,Mouse
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Mitochondrion. Peroxisome.
- 其它名称2 arylpropionyl CoA epimerase antibody
2 methylacyl CoA racemase antibody
2-methylacyl-CoA racemase antibody
Alpha methylacyl CoA racemase antibody
Alpha methylacyl Coenzyme A racemase antibody
Alpha methylacyl-CoA racemase deficiency, included antibody
Alpha-methylacyl-CoA racemase antibody
Amacr antibody
AMACR deficiency, included antibody
AMACR_HUMAN antibody
CBAS4 antibody
Da1-8 antibody
EC 5.1.99.4 antibody
Macr1 antibody
Methylacyl CoA racemase alpha antibody
RACE antibody
RM antibody
more
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应用
WB: 1:500
ICC: 1:50-1:100
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of AMACR on different lysates using anti-AMACR antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse liver tissue
Lane 2: SiHa
Lane 3: SW480
Lane 4: Human kidney tissue
Fig2: ICC staining AMACR in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AMACR antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-AMACR antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AMACR antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of PC-3M cells with AMACR antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
