Anti-IDO antibody

Anti-IDO antibody

• 概述

• 产品描述Indoleamine 2,3-dioxygenase (IDO) is an IFN-γ inducible enzyme that catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. The gene encoding human IDO maps to chromosome 8p12-p11. IDO, also known as INDO, is an important modulator of immunological responses and protects allogeneic concepti from alloreactive maternal lymphocytes. IDO mediates an interesting inhibitory effect of HeLa cells co-cultured with human PBLs. The ILN-2-induced proliferation response of PBLs is diminished in the presence of HeLa cells while an IDO inhbitor negates this effect. Flow cytometric analysis indicates both mature and immature CD123-positive dentritic cells suppress T cell activity using IDO. IDO-transfected cells co-cultured with T cells reduces T cell proliferation. Additionally, adopted transfer of donor T cells reduces donor T cell numbers in IDO-transgenic mice. The pharmacological or genetic manipulation of IDO may be useful for supressing undesirable T cell response.
• 产品名称Anti-IDO antibody
• 分子量45 kDa
• 种属反应性Human,Mouse
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型兔多抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG
• 纯化方式Protein affinity purified
• 亚细胞定位Cytoplasm.

• 应用

  WB: 1:500-1:2,000
  ICC: 1:100-1:500
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of IDO on human placenta tissue lysate using anti-IDO antibody at 1/500 dilution.

   

  

  Fig2: ICC staining IDO in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining IDO in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IDO antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IDO antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of HUVEC cells with IDO antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

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