Anti-DSPP antibody

Anti-DSPP antibody

• 概述

• 产品描述DSP may be an important factor in dentinogenesis. DPP may bind high amount of calcium and facilitate initial mineralization of dentin matrix collagen as well as regulate the size and shape of the crystals.
• 产品名称Anti-DSPP antibody
• 分子量47/129 KDa
• 种属反应性Human
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human Dentin sialoprotein 31-130/1301
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Secreted, extracellular space, extracellular matrix.
• 其它名称Dentin phosphophoryn
  Dentin phosphoprotein
  dentin phosphoryn
  Dentin sialophosphoprotein
  Dentin sialophosphoprotein precursor
  Dentin sialoprotein
  dentinogenesis imperfecta 1
  DFNA39
  DGI1
  DMP3
  DPP
  DSP
  DSPP_HUMAN
  DTDP2.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-DSPP Polyclonal Antibody, Unconjugated1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

   

  

  Fig2: Sample:
  EC-9706(Human) Cell Lysate at 30 ug
  MCF-7(Human) Cell Lysate at 30 ug
  Primary: Anti-DSPPat 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 47/129 kD
  Observed band size: 129 kD

   

  

  Fig3: Sample:
  LOVO(Human) Cell Lysate at 30 ug
  293T(Human) Cell Lysate at 30 ug
  Primary: Anti-DSPat 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 47/129 kD
  Observed band size: 67/129 kD

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！