Anti-ZAR1L antibody
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概述
- 产品名称Anti-ZAR1L antibody
- 分子量36 KDa
- 种属反应性Human
- 验证应用IHC-P,WB
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human ZAR1L 51-150/321
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 其它名称RP11-37E23.4
ZAR1-like protein
ZAR1L
ZAR1L_HUMAN
ZAR2
Zygote arrest 1 like.
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Sample:A549 (Human)cell Lysate at 40 ug
Primary: Anti-ZAR1at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 36kD
Observed band size: 36kD
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ZAR1L) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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