Anti-ADRB3 antibody

Anti-ADRB3 antibody

• 概述

• 产品描述Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis.
• 产品名称Anti-ADRB3 antibody
• 分子量44 KDa
• 种属反应性Human,Mouse,Rat,Dog,Pig,Sheep
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human ADRB3 310-408/408
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cell membrane; Multi-pass membrane protein.
• 其它名称beta 3 Adrenergic Receptor
  beta3-adrenergic receptor
  beta3-AR
  adrenergic
  beta-3- receptor
  ADR B3
  ADRB 3
  ADRB3R
  Adrenergic beta 3 receptor
  B3AR
  Beta 3 adrenoceptor
  Beta 3 adrenoreceptor
  Beta 3AR
  B3AR
  BETA3AR
  ADRB3_HUMAN.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Tissue/cell: Rat adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-ADRB3 Polyclonal Antibody, Unconjugated1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

   

  

  Fig2: Sample:
  K562(Human) Cell Lysate at 40 ug
  Primary: Anti-beta 3 Adrenergic Receptor  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 44 kD
  Observed band size: 54 kD

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！