Anti-ATPase Na+/ K+ beta 2 antibody
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概述
- 产品描述This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known.
- 产品名称Anti-ATPase Na+/ K+ beta 2 antibody
- 分子量33 KDa
- 种属反应性Human,Mouse,Rat,Pig,Cow,Horse,Rabbit,Guinea Pig
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human ATP1b2 201-290/290
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Membrane; Single-pass type II membrane protein
- 其它名称adhesion molecule on glia
Na+K+ATPase
AMOG
AT1B2
AT1B2_HUMAN
ATP1B2
ATPase Na+/K+ transporting beta 2 polypeptide
ATPB2
ATPB2S
MGC93648
Na+/K+ -ATPase beta 2 subunit
Na, K ATPase beta 2 polypeptide
RATATPB2S
sodium potassium ATPase subunit beta 2 (non-catalytic)
sodium pump subunit beta 2
sodium/potassium dependent ATPase beta 2 subunit
Sodium/potassium dependent ATPase subunit beta 2
sodium/potassium transporting ATPase beta 2 chain
sodium/potassium transporting ATPase subunit beta 2
Sodium/potassium-dependent ATPase subunit beta-2
Sodium/potassium-transporting ATPase subunit beta-2.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Tissue/cell: mouse colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ATP1b2/Na+K+ATPase Polyclonal Antibody, Unconjugated1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Fig2: Sample: NIH/3T3(mouse) Cell Lysate at 40 ug
Primary: Anti-ATPaseNa＋/K＋ beta2 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
Fig3: Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AMOG Polyclonal Antibody, Unconjugated1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Fig4: Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-ATPase Na+/ K+ beta 2 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
