Anti-APC antibody
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概述
- 产品描述The adenomatous polyposis syndromes, familial adenomatous polyposis (FAP) and Gardner's syndrome (GS), are characterized by numerous adenomatous polyps throughout the entire colon. These polyps invariably progress to colon cancer in addition to other extracolonic manifestations. The cloning of the APC gene revealed a ubiquitously expressed protein, 2,843 amino acids in length, which is frequently mutated in patients suffering from FAP and GS. APC has been found to be associated with structural components of intracellular junctions. β-catenin and γ-catenin (also called plakoglobin), are involved in the regulation of cellular adhesion. APC and E-cadherin compete for binding to specific internal regions of both β- and γ-catenin. Interactions between cytoskeleton and the APC, E-cadherin, β/γ catenin complex are mediated by α-catenin.
- 产品名称Anti-APC antibody
- 分子量Predicted band size 312 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Peptide
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Cell junction. Cytoplasm. Cytoskeleton. Membrane.
- 其它名称Adenomatous Polyposis Coli antibody
Adenomatous polyposis coli protein antibody
Apc antibody
APC_HUMAN antibody
CC1 antibody
Deleted in polyposis 2.5 antibody
DP2 antibody
DP2.5 antibody
DP3 antibody
FAP antibody
FPC antibody
GS antibody
Protein APC antibody
more
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应用
ICC: 1:50-1:200
IHC-P: 1:100-1:200
FC: 1:50-1:100
WB: 1:500
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Fig1: ICC staining APC in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig2: ICC staining APC in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining APC in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-APC antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-APC antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-APC antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-APC antibody. Counter stained with hematoxylin.
Fig8: Flow cytometric analysis of SH-SY-5Y cells with APC antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the seconda
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