Anti-B7-H4(VTCN1) antibody
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概述
- 产品描述T cell activation and immune function are regulated by the innate immune system through positive and negative costimulatory proteins. One such protein, B7-H4 (B7-homolog 4, also designated VTCN1), belongs to the B7 Immunoglobulin superfamily of ligand-lymphocyte interacting proteins. Expressed primarily on the membrane of lymphoid cells, B7-H4 is an immunoinhibitory protein that interacts with receptors on the surface of T lymphocytes, thus mediating cellular and humoral immune responses. Overexpression of the B7-H4 protein is associated with certain malignancies, including ovarian and breast cancer, as its interaction with T cells supresses tumor-associated immunity. Current research suggests that, similar to Mucin 16 (CA125), B7-H4 may be a useful biomarker for the early detection of ovarian cancer.
- 产品名称Anti-B7-H4(VTCN1) antibody
- 分子量31/21/10 kDa (Predicted band size)
- 种属反应性Human,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein with human B7-H4 aa 50-220.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Cell membrane.
- 其它名称B7 family member, H4 antibody
B7 H4 antibody
B7 homolog 4 antibody
B7 superfamily member 1 antibody
B7 superfamily, member 1 antibody
B7-H4 antibody
B7h.5 antibody
B7h4 antibody
B7S1 antibody
B7x antibody
BC032925 antibody
Immune costimulatory protein B7-H4 antibody
Immune costimulatory protein B7H4 antibody
MGC41287 antibody
PRO1291 antibody
Protein B7S1 antibody
RP11 229A19.4 antibody
T cell costimulatory molecule B7x antibody
T-cell costimulatory molecule B7x antibody
V set domain-containing T cell activation inhibitor 1 antibody
V-set domain-containing T-cell activation inhibitor 1 antibody
VCTN1 antibody
Vtcn1 antibody
VTCN1_HUMAN antibody
more
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应用
WB: 1:500-1:2000
ICC: 1:1,000
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of B7-H4(VTCN1) on SKBR3 cell and human colon tissue lysates using anti-B7-H4(VTCN1) antibody at 1/500 dilution.
Fig2: ICC staining B7-H4(VTCN1) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-B7-H4(VTCN1) antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-B7-H4(VTCN1) antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-B7-H4(VTCN1) antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of MCF-7 cells with B7-H4(VTCN1) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
