Anti-Rae1 antibody
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概述
- 产品描述Mrnp41 (mRNA-binding protein, 41-KD), also known as Rae1 protein homolog and mRNA export factor, is a 368 amino acid protein that binds mRNA and is involved in nucleocytoplasmic transport. Though characterized in both the nucleus and cytoplasm, mrnp41 is primarily localized to the nuclear pore complex in the nuclear envelope. Mutations in mrnp41 may result in the accumulation of poly(A)-containing mRNA in the nucleus, further supporting the role of mrnp41 as a primary nuclear exporter of mRNA. Along with Nup98, mrnp41 has been shown to regulate E-cadherin, an activating subunit of the anaphase-promoting complex complex, which results in the prevention of securin degradation, therefore suggesting that mrnp41 may play a potential role in maintaining euploidy. Also, during mitosis, both mrnp41 and NuMA have been shown to be required for bipolar spindle formation.
- 产品名称Anti-Rae1 antibody
- 分子量41 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,FC
- 抗体类型兔多抗
- 免疫原Synthetic peptide within human Rae1 aa 330-368.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Nucleus. Cytoskeleton.Cytoplasm.
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应用
WB: 1:500-1,000
ICC: 1:50-1:200
FC: 1:50-1:200
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Fig1: Western blot analysis of Rae1 on different lysates using anti-Rae1 antibody at 1/500 dilution.
Positive control:
Lane 1: PC-3
Lane 2: Mouse testis tissue
Lane 3: Rat testis tissue
Fig2: ICC staining Rae1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Rae1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Rae1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Flow cytometric analysis of LOVO cells with Rae1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Flow cytometric analysis of LOVO cells with Rae1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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