Anti-NQO1 antibody
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概述
- 产品描述NQO1, a 274 amino acid protein, is ubiquitously expressed, but the expression level varies among tissues. NQO1 gene expression is coordinately induced in response to xenobiotics, antioxidants, heavy metals and radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression and coordinated induction of NQO1. ARE activation by tert-butylhydroquinone is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B and Jun-D bind to the ARE and regulate expression and induction of NQO1 gene. Maf-Maf homodimers and possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE and do not repress NQO1 transcription.
- 产品名称Anti-NQO1 antibody
- 分子量31 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein within human NQO1 1-250 aa.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Cytoplasm.
- 其它名称Azoreductase antibody
Cytochrome b 5 reductase antibody
DHQU antibody
DIA 4 antibody
DIA4 antibody
Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody
Diaphorase (NADH/NADPH) antibody
Diaphorase 4 antibody
Dioxin inducible 1 antibody
DT diaphorase antibody
DT-diaphorase antibody
DTD antibody
Menadione reductase antibody
NAD(P)H dehydrogenase [quinone] 1 antibody
NAD(P)H dehydrogenase quinone 1 antibody
NAD(P)H menadione oxidoreductase 1 dioxin inducible antibody
NAD(P)H quinone dehydrogenase 1 antibody
NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 antibody
NAD(P)H:menadione oxidoreductase 1 antibody
NAD(P)H:Quinone acceptor oxidoreductase type 1 antibody
NAD(P)H:quinone oxidoreductase 1 antibody
NAD(P)H:quinone oxireductase antibody
NMOR 1 antibody
NMOR I antibody
NMOR1 antibody
NMORI antibody
NQO 1 antibody
NQO1 antibody
NQO1_HUMAN antibody
Phylloquinone reductase antibody
QR 1 antibody
QR1 antibody
Quinone reductase 1 antibody
more
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应用
WB: 1:500-1:1,000
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of NQO1 on different cell lysates using anti-NQO1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Siha
Lane 2: A549
Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-NQO1 antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-NQO1 antibody. Counter stained with hematoxylin.
Fig4: Flow cytometric analysis of Siha cells with NQO1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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