Anti-CD15 antibody

Anti-CD15 antibody

• 概述

• 产品描述May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.

• 产品名称Anti-CD15 antibody

• 分子量58 KDa

• 种属反应性Human,Mouse,Rat

• 验证应用WB,IHC-P,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human CD15 251-295/433

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi.

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• ​其它名称Fut4
  SSEA-1
  3-FAL
  Alpha (1,3) fucosyltransferase
  Alpha 13 fucosyltransferase FucT
  EC 2.4.1.
  ELAM 1 ligand fucosyltransferase
  ELAM ligand fucosyltransferase
  ELAM1 ligand fucosyltransferase
  ELFT
  FCT3A
  Fuc TIV
  Fucosyltransferase 4 alpha 1 3 fucosyltransferase myeloid specific
  Fucosyltransferase 4
  FucT IV
  FUCTIV
  FUT4
  Galactoside 3 L fucosyltransferase
  Lewis X
  LeX
  SSEA 1
  SSEA1
  Stage specific embryonic antigen 1
  FUC-TIV.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:1μg/Test

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  Fig1: Overlay histogram showing HL 60 cells stained with (Green line).
  The cells were fixed with 90% methanol (5 min) and then permeabilized with 0.01M PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody for 30 min at 22℃. The secondary antibody used was fluorescein isothiocyanate goat anti-rabbit IgG (H+L) (bs- 0295G-FITC , Brillant blue line) at 1/200 dilution for 30 min at 22℃. Isotype control antibody was rabbit IgG (polyclonal,bs-0295P,Orange line) (1μg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of 20,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

   

  

  Fig2: Sample:A549 Cell (Human) Lysate at 40 ug
  Primary: Anti-CD15at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 58kD
  Observed band size: 63kD

   

  

  Fig3: Paraformaldehyde-fixed, paraffin embedded (Human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CD15) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig4: Blank control: Mouse spleen.
  Primary Antibody (green line): Rabbit Anti-CD15 /FITC Conjugated antibody 
  Dilution: 1μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG-FITC .
  Protocol
  The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！