Anti-TMEM163 antibody
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概述
- 产品描述Previously, it was shown that purified rodent Tmem163 protein reconstituted in liposomes forms a dimer and transports zinc in a proton-dependent manner. It was also shown that Tmem163's ability to bind and transport zinc becomes inactivated upon alanine substitution of two species-conserved aspartate residues at positions 123 and 127. Thus, the data indicate that these double aspartate residues are zinc binding sites, but that they do not affect Tmem163 dimerization. Note, however, that this particular study did not address the question of whether the protein serves to transport zinc in or out of living cells. TMEM163 may play a crucial role in cellular zinc homeostasis by extruding cytoplasmic zinc ions to the extracellular environment. Noteworthy is that TMEM163 is unique in that it has a short C-terminus region, whereas the human ZNT family of efflux transporters have typically long C-terminus regions. Despite such structural difference, data from both functional and phylogenetic studies of TMEM163 suggest that it should now be referred to as ZNT11 protein and its gene named as SLC30A11.
- 产品名称Anti-TMEM163 antibody
- 分子量Predicted band size 31 kDa.
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Synthetic peptide within human TMEM163 aa 50-100.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Early endosome membrane, synaptic vesicle membrane.
- 其它名称
- DC29 antibody
- DKFZp566N034 antibody
- DKFZp666J217 antibody
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应用
WB:1:500-1:2,000
ICC:1:50-1:200
FC:1:50-1:100
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Fig1: Western blot analysis of TMEM163 on human small intestine tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: ICC staining of TMEM163 in 293 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Fig3: ICC staining of TMEM163 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-87, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Fig4: Flow cytometric analysis of TMEM163 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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