Anti-PFKFB2/PFK2 antibody

Anti-PFKFB2/PFK2 antibody

• 概述

• 产品描述6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 is an enzyme that in humans is encoded by the PFKFB2 gene. The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. PFKFB2 has been shown to interact with YWHAQ.

• 产品名称Anti-PFKFB2/PFK2 antibody

• 分子量58 KDa

• 种属反应性Human,Mouse,Rat,Chicken,Dog

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human PFKFB2 51-150/505

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式Affinity purified by Protein A

• 亚细胞定位PC-3 Cell Lysate, Rat brain tissue, Rat kidney tissue, Mouse brain tissue.

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• ​其它名称6 phosphofructo 2 kinase/fructose 2 6 biphosphatase 2
  6-bisphosphatase
  6-P2ase 2
  6-P2ASE heart-type isozyme
  6PF 2 K/Fru 2 6 P2ASE heart type isozyme
  6PF-2-K/Fru-2
  F262_HUMAN
  Fructose 2 6 bisphosphatase cardiac isozyme
  Fructose-2
  PFK 2/FBPase 2
  PFK/FBPase 2
  PFKFB cardiac
  PFKFB2.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

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  Fig1: Sample:
  PC-3 Cell Lysate at 40 ug
  HepG2 Cell Lysate at 40 ug
  Primary: Anti- PFKFB2/PFK2 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 58 kD
  Observed band size: 62 kD

   

  

  Fig2: Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-PFKFB2 Polyclonal Antibody, Unconjugated  1:500, overnight at 4℃, followed by conjugation to the secondary antibody (SP-0023) and DAB (C-0010) staining

   

  

  Fig3: Tissue/cell: Rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum, C-0005) at 37℃ for 20 min;
  Incubation: Anti-PFKFB2 Polyclonal Antibody, Unconjugated 1:500, overnight at 4℃, followed by conjugation to the secondary antibody (SP-0023) and DAB (C-0010) staining

   

  

  Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH 6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PFKFB2) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit (Rabbit) (sp-0023) instructionsand DAB staining.

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！