Anti-CD23 antibody

Anti-CD23  antibody

• 概述

• 产品描述Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).
• 产品名称Anti-CD23 antibody
• 分子量36 KDa
• 种属反应性Human,Mouse,Rat,Horse,Rabbit
• 验证应用WB,FC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human CD23 221-321/321
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cell membrane; Single-pass type II membrane protein. Secreted. Note=Also exists as a soluble excreted form.
• 其它名称Fc EpsilonR II
  FcεRII
  Blast 2
  Blast2
  C type lectin domain family 4 member J
  C-type lectin domain family 4, member J
  CD 23
  CD 23A
  CD23 antigen
  CD23A
  CLEC 4J
  CLEC4J
  Fc epsilon RII
  Fc fragment of IgE low affinity II receptor for
  Fc fragment of IgE, low affinity II, receptor for (CD23)
  Fc of IgE, low affinity II, receptor for (CD23)
  Fc receptor IgE low affinity II alpha polypeptide
  Fc receptor, IgE, low affinity II, alpha polypeptide, isoform CRA_a
  FCE 2
  FCE2
  FCER 2
  FCER2
  FCER2A
  IgE binding factor
  IgE receptor lymphocyte
  IgE-binding factor
  IGEBF
  Immunoglobulin E binding factor
  Immunoglobulin E receptor, low affinity II
  Low affinity immunoglobulin epsilon Fc receptor
  Low affinity immunoglobulin epsilon Fc receptor membrane bound form
  Low affinity immunoglobulin epsilon Fc receptor soluble form
  Ly-42
  Ly42
  Lymphocyte antigen CD23
  Lymphocyte IgE receptor
  MGC93219.
  more

• 应用

  WB:1:500-2000
  FC:1μg/Test

•  

  

  Fig1: Blank control(blue):Raji cells (fixed with 2% paraformaldehyde (10 min)).
  Primary Antibody:Rabbit Anti- CD23 antibody Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

   

  

  Fig2: Sample:
  Spleen (Mouse) Lysate at 40 ug
  Primary: Anti- CD23at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 36 kD
  Observed band size: 50/40 kD

   

  

  Fig3: Sample:
  293T Cell (Human) Lysate at 30 ug
  Primary: Anti- CD23 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 36 kD
  Observed band size: 50/40 kD

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！